ERYTHROCYTE PRESERVATION

Erythrocyte preservation. I. The relation of the storage lesion to in vivo erythrocyte senescence.

Erythrocytes stored in an acid-citrate-dextrose preservative (ACD) at 4 to 7° C. undergo morphological and biochemical alterations. The cell becomes more sphere-shaped with an associated increase in osmotic and mechanical fragilities, the glycolytic rate and organic phosphate compounds are decreased, inorganic phosphate is increased, and the differential between cell potassium and sodium is dec...

Preservation of erythrocyte ghost ultrastructure achieved by various fixatives.

We have evaluated the quality of ultrastructural preservation of erythrocyte ghosts achieved under various electron microscopic preparative conditions. Initially, negative staining was used to monitor gross morphology of the ghosts. Of several negative stains used (phosphotungstate, silicotungstate, ammonium molybdate, and uranyl acetate), all but uranyl acetate resulted in fragmentation of mem...

Erythrocyte preservation. III. The reversibility of the storage lesion.

Preservation of the Lipids of the Human Erythrocyte Stroma during Fixation and Dehydration for Electron Microscopy

Recent reports of lipid and/or protein losses from biological material during fixation processes (Luft and Wood, 1963; Ashworth et al., 1966; Korn and Weisman, 1966; Morgan and Huber, 1967; Stein and Stein, 1967 a, b, 1968; Buschmann and Taylor, 1968; Ongun et al., 1968) emphasize the necessity for basing the interpretation of electron micrographs upon knowledge of the final composition of the ...

Erythrocyte fragility in hyperthyroid patients

  Mild anaemia among patients with hyperthyroidism, reduction in intracranial zinc content of erythrocytes and anti- haemolysis of whole blood of patients with Graves, disease under laboratory Condition are the facts which indicate that although erythrocytes are devoid of receptors for thyroid hormones, they are affected by the excess of these hormones. In order to find out the reasons for suc...